Over the past twenty years, the Digital Biology Lab (DBL) has witnessed rapid progress in bioinformatics and computational biology, and it has achieved significant milestones in research, education, and collaboration. The DBL has shown strong adaptability by identifying new opportunities and branching into emerging fields. It has developed numerous innovative algorithms, software packages, and information systems that provide effective tools for addressing a wide range of biological and medical challenges. In addition, the DBL is actively involved in extensive data analysis and modeling for biomedical studies. As we have reached this significant twenty-year milestone, we reflect on our past accomplishments and look forward to future endeavors, celebrating the collective efforts and continuous commitment of our team.